[ Optical Images ] BDCC-OP-0044_Simple Signal Amplification of Immunofluorescence using Anti-fluorophore Antibodies

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Field-specification
Package Contents
Date of SOP Creation
10.04.2024
Title
BDCC-OP-0044_Simple Signal Amplification of Immunofluorescence using Anti-fluorophore Antibodies
Authors
Youngbin Yoon
Jae-Byum Chang


Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology;jbchang03@kaist.ac.kr
Scope and Applicability
This protocol describes a method for enhancing fluorescence signals in biological samples for X10-STED super-resolution microscopy. The key focus is on pre-expansion staining using Alexa 488-conjugated antibodies and subsequent amplification with anti-Alexa 488 antibodies, enabling high-resolution imaging for various biological applications, including neuronal cultures, organelle structures, or isolated vesicles.
Keywords
Expansion microscopy, Anti-Alexa 488, Signal amplification, Super-resolution microscopy, STED
Instruments
Confocal/STED microscope, Autoclave (for heat denaturation), Centrifuge (for vesicle preparation), Humidified chamber (for gelation)