Optical Images
Our mission is to provide basic science and clinical researchers with standardized imaging protocols, and state of the art image tools they need to explore the hidden structures and dynamic processes within cells, organelles, tissues, and living organisms. Our team specializes in the curation and analysis of light microscope images and fluorescence-labeled images, particularly those obtained from light microscopes for observing live cells, tissues, histological sections, and various other biological specimens. Our expertise lies in imaging cellular and subcellular structures, tracking the movement of fluorescently labeled molecules, and examining tissues and live organisms in three dimensions.
Specifically, we offer technical support to Korean researchers, aiming to enhance their imaging capabilities. Our assistance includes facilitating superior image acquisition, precise image analysis, and effective archiving of vast image datasets. Furthermore, we support the efficient reuse and sharing of these images. For image acquisition, we provide a standard operating procedure (SOP) that encompasses various imaging techniques, from live cell and multiplexed spatial proteomics imaging to super-resolution large-volume imaging using tissue expansion. Our services ensure quantitative assessments of image quality, including evaluations of signal-to-noise ratios and resolution. Additionally, we offer tailored consultations, assisting researchers with troubleshooting their individual imaging experiments, recommending optimal imaging methods, and even suggesting potential research collaborators.
Multiphoton luminescence (MPL) imaging, one of the most powerful optical imaging techniques, employs near-infrared light to observe the biological substances since these molecules can absorb two or more NIR photons simultaneously and produce luminescence. In MPL microspectroscopy, femtosecond NIR excitation induces the least degree of photodamage in cells, allowing for the study of dynamic compositional changes in cells and organs, as well as sophisticated responses in physiological environments. Furthermore, because NIR light can penetrate deep into tissue, MPL can perform 4-dimensional intravital imaging, enabling in-blood monitoring of target materials as well as tracking of hopping cells in organs or tissues of living animals. On this platform, MPL images provide information of natural life that are explored deeper in space for a longer period of time in biological microenvironments.
Kidney tissue (excitation:830nm)