People
Jae-Byum Chang, Ph.D.
KAIST, Associate Professor
Principal Investigator of BDCC
jbchang03@kaist.ac.kr
Research Interests
Super-resolution optical imaging, polymer, hydrogel, and nanofabrication
Fluorescence microscopy has been widely used in neuroscience and cancer research.
However, it still has two limitations: (1) the number of fluorophores that can be simultaneously used is limited to four, and (2) achieving super-resolution imaging over the whole organism is not easy. Prof. Jae-Byum Chang develops diverse techniques to enable the super-resolution multiplexed imaging over entire organism.
First, he has developed various multiplexed imaging techniques, which enable simultaneous imaging of more than 30 proteins from diverse specimens. The second technique he has developed is whole-body expansion microscopy, which enables super-resolution imaging of all anatomical structures of whole vertebrates, especially zebrafish larvae.
Recent Publications
- PICASSO allows ultra-multiplexed fluorescence imaging of spatially overlapping proteins without reference spectra measurements, Nature Communications volume 13, Article number: 2475 (2022)
- Multiscale Functional Metal Architectures by Antibody-Guided Metallization of Specific Protein Assemblies in Ex Vivo Multicellular Organisms, Adv.Mater. Volume34, Issue35 2200408 (2022)
- Super-Resolution Three-Dimensional Imaging of Actin Filaments in Cultured Cells and the Brain via Expansion Microscopy, ACS Nano 14, 14999-15010 (2020)
- Iterative expansion microscopy, Nature Methods volume 14, pages593–599 (2017)
- Design rules for self-assembled block copolymer patterns using tiled templates, Nature Communications volume 5, Article number: 3305 (2014)
Participating Faculty and Researchers
-
In Cho, Ph.D.
Postdoctoral Researcher
Graduate Students
- Chang Woo Song
- Email : scwoo@kaist.ac.kr
- Research Interests : biomaterials, biotemplating, catalysis
- Hyunwoo Kim
- Email : hkim151@kaist.ac.kr
- Research Interests : Spatial proteomics, Multiplexed cyclic imaging, Fluorescence microscopy
- Dae-Hyeon Song
- Email : dhsong9518@kaist.ac.kr
- Research Interests : biotemplating, biomimetic, biomolecular imaging
- Jueun Sim
- Email : jueunsim@kaist.ac.kr
- Research Interests : Expansion microscopy, super-resolution imaging. Nanoscale imaging of the whole vertebrate using expansion microscopy
- Hye Been Koo
- Email : hbkoo@kaist.ac.kr
- Research Interests : polymer, soft robotics
- Woonggi La
- Email : fkdndrl@kaist.ac.kr
- Research Interests : super-resolution imaging, biotemplating
- Chan Kang
- Email : ckang191@kaist.ac.kr
- Research Interests : biotemplating
- Youngbin Yoon
- Email : youngbin135@kaist.ac.kr
- Research Interests : Hydrogel
- Hoeyun Jung
- Email : hoiyunj@kaist.ac.kr
- Research Interests : biotemplating
- Nakhyun Kim
- Email : naktal0204@kaist.ac.kr
- Research Interests : biotemplating
Researchers
- Chan E Park
- Email : chanchane@kaist.ac.kr
- Research Interests : Expansion microscopy (cell, tissue)
- Seung Jae Lee
- Email : sjlee23@kaist.ac.kr
- Research Interests : Tissue imaging
- Hyejin Shin
- Email : shinhj0730@kaist.ac.kr
- Research Interests : Multiplexed imaging, Fluorescence microscopy
Research Interests
- Molecular imaging, cell imaging, expansion microscopy, RNA imaging
Research Interests
- Multiplexed imaging, super-resolution imaging
Recent Publications
- PICASSO allows ultra-multiplexed fluorescence imaging of spatially overlapping proteins without reference spectra measurements, Nature Communications volume 13, Article number: 2475 (2022)